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KMID : 0379119970250030219
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Korean Journal of Mycology
1997 Volume.25 No. 3 p.219 ~ p.225
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Classification of Korean Lentinula edodes Strains by Random Amplified Polymorphic DNA (RAPD) Markers
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이원규/Lee, Won Kyu
이창근/김세권/강호덕/변병호/민두식/박원철/Yi, Chang Keun/Kim, Se Kwon/Kang, Ho Duck/Byun, Byung Ho/Min, Du Sik/Bak, Won Chull
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Abstract
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Random Amplified Polymorphic DNA (RAPD) assay was used to identify seven typical Lentinula edodes (Berk.) Pegler strains isolated in Korea. Twenty primers from OPA-01 to OPA-20 were applied to generate the recognition of L. edodes strains. Out of 20 primers, nine primers showed efficient RAPD patterns to classify the 7 strains tested, but the rest eleven primers were not useful to be used. Even though there was no single primer that could classify all of the strains, any combination of two primers among the nine primers could identify the strains tested. Thus, RAPD assay turned out to be very precise method for classifying L. edodes strains.
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